Hokkaido University Institute for Chemical Reaction Design and Discovery

Research Outline

At ICReDD, I am in charge of the storage, management, and analysis of research data, along with the management of data storage devices and measurement and analysis equipment.
In my research work, I am studying how to efficiently produce valuable substances (such as bioactive proteins and antibodies) by animal cell culture. For example, I have been working on various aspects, including promoting proliferation through nutrient supplementation considering cellular clock gene expression and constructing robust cells capable of withstanding rigorous stirred cultivation conditions.

Representative Research Achievements

• Effect of combination of residual glucose concentration and subsequent increment by temporal glucose feeding on oscillation of clock gene Per2 expression
  Fukaura E., Kiriaki K., Kayier M., Fujiwara M., Takagi M. Cytotechnology, 2022, 74, 193-200.
  DOI: 10.1007/s10616-021-00505-z
• Polymer fraction including exosomes derived from Chinese hamster ovary cells promoted their growth during serum-free repeated batch culture
  Takagi M., Jimbo S., Oda T., Goto Y., Fujiwara M. J. Biosci. Bioeng., 2021, 131, 183-189.
  DOI: 10.1016/j.jbiosc.2020.09.011
• Comparison of percentage of CD90-positive cells and osteogenic differentiation potential between mesenchymal stem cells grown on dish and nonwoven fabric
  Fu B., Fujiwara M., Takagi M. Cytotechnology, 2020, 72, 433-444.
  DOI: 10.1007/s10616-020-00390-y
• Protocol of mesenchymal stem cell inoculation to nonwoven fabric scaffold
  Fu B., Yamada K., Fujiwara M., Iwai R., Takagi M. Cytotechnology, 2019, 71, 743-750.
  DOI: 10.1007/s10616-019-00320-7
• Ex vivo cartilage defect model for the evaluation of cartilage regeneration using mesenchymal stem cells
  Iwai R., Fujiwara M., Wakitani S., Takagi M. J. Biosci. Bioeng., 2011, 111, 357-364.
  DOI: 10.1016/j.jbiosc.2010.11.001