About the Research
Research Theme
Research Data Management, Animal Cell Culture Engineering
Keyword
Data management, animal cell culture, valuable substance production
Research Outline
At ICReDD, I am in charge of the storage, management, and analysis of research data, along with the management of data storage devices and measurement and analysis equipment.
In my research work, I am studying how to efficiently produce valuable substances (such as bioactive proteins and antibodies) by animal cell culture. For example, I have been working on various aspects, including promoting proliferation through nutrient supplementation considering cellular clock gene expression and constructing robust cells capable of withstanding rigorous stirred cultivation conditions.
Representative Research Achievements
- Effect of combination of residual glucose concentration and subsequent increment by temporal glucose feeding on oscillation of clock gene Per2 expression
Fukaura E., Kiriaki K., Kayier M., Fujiwara M., Takagi M. Cytotechnology, 2022, 74, 193-200.
DOI: 10.1007/s10616-021-00505-z - Polymer fraction including exosomes derived from Chinese hamster ovary cells promoted their growth during serum-free repeated batch culture
Takagi M., Jimbo S., Oda T., Goto Y., Fujiwara M. J. Biosci. Bioeng., 2021, 131, 183-189.
DOI: 10.1016/j.jbiosc.2020.09.011 - Comparison of percentage of CD90-positive cells and osteogenic differentiation potential between mesenchymal stem cells grown on dish and nonwoven fabric
Fu B., Fujiwara M., Takagi M. Cytotechnology, 2020, 72, 433-444.
DOI: 10.1007/s10616-020-00390-y - Protocol of mesenchymal stem cell inoculation to nonwoven fabric scaffold
Fu B., Yamada K., Fujiwara M., Iwai R., Takagi M. Cytotechnology, 2019, 71, 743-750.
DOI: 10.1007/s10616-019-00320-7 - Ex vivo cartilage defect model for the evaluation of cartilage regeneration using mesenchymal stem cells
Iwai R., Fujiwara M., Wakitani S., Takagi M. J. Biosci. Bioeng., 2011, 111, 357-364.
DOI: 10.1016/j.jbiosc.2010.11.001
Publications
2024
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Application of Bacterial-Derived Long Cellulose Nanofiber to Suspension Culture of Mammalian Cells as a Shear Protectant
, H. Tsujisaki, M. Fujiwara, H. Ando, Y. Sato, T. Ishida, H. Tani, K. Tajima, International Journal of Biological Macromolecules, 2024, 280, part 3, 135938
DOI: 10.1016/j.ijbiomac.2024.135938
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Optimizing Crystal Transitions in Low-Temperature, Low-Concentration NaOH Solutions to Prepare Cellulose I and II Composite Materials
, T. Isono, M. Fujiwara, T. Sato, H. Tani, T. Erata, K. Tajima, Polymer Journal, 2024, 56, 939–943
DOI: 10.1038/s41428-024-00928-3
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Elucidating the Structural Changes of Cellulose Molecules and Dynamics of Na Ions During the Crystal Transition from Cellulose I to II in Low Temperature and Low Concentration NaOH Solution
, S. Nomura, T. Isono, S. I. Sato, M. Fujiwara, T. Satoh, H. Tani, T. Erata, K. Tajima, Carbohydrate Polymers, 2024, 332,
DOI: 10.1016/j.carbpol.2024.121907
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Crystallinity Improvements in Cellulose II after Multiple Posttreatment Cycles with a Dilute NaOH Solution
, S. Nomura, T. Isono, M. Fujiwara, T. Satoh, H. Tani, T. Erata, K. Tajima, Polymer Journal, 2024, ,
DOI: 10.1038/s41428-024-00890-0